ELISPOT Refinement Using Spot Morphology for Assessing Host Responses to Tuberculosis
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ELISPOT Refinement Using Spot Morphology for Assessing Host Responses to Tuberculosis
Tuberculosis is a global health problem. The Mycobacterium bovis Bacille Calmette Guerin (BCG) vaccine has variable efficacy (0-80%) so there is a drive to develop novel vaccines. The cytokine, interferon gamma (IFNγ), is an essential component of the protective response to M. tuberculosis (M. tb) infection and is also produced in response to BCG vaccination. Induction of an IFNγ response is us...
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A number of different interferon-gamma ELISpot protocols are in use in laboratories studying antigen-specific immune responses. It is therefore unclear how results from different assays compare, and what factors most significantly influence assay outcome. One such difference is that some laboratories use a short in vitro stimulation period of cells before they are transferred to the ELISpot pla...
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BACKGROUND Very little longitudinal information is available regarding the performance of T cell-based tests for Mycobacterium tuberculosis infection. To address this deficiency, we conducted a longitudinal assessment of the enzyme-linked immunosorbent spot test (ELISPOT) test in comparison to the standard tuberculin skin test (TST). METHODS AND FINDINGS In tuberculosis (TB) contacts we repea...
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ژورنال
عنوان ژورنال: Cells
سال: 2012
ISSN: 2073-4409
DOI: 10.3390/cells1010005